Real-time PCR assay for the diagnosis of pleural tuberculosis

Real-time PCR assay for the diagnosis of pleural tuberculosis

INTRODUCTION The diagnosis of pleural tuberculosis requires an invasive and time-consuming reference method. Polymerase chain reaction (PCR) is rapid, but validation in pleural tuberculosis is still weak. OBJECTIVE To establish the operating characteristics of real-time polymerase chain reaction (RT-PCR) hybridization probes for the diagnosis of pleural tuberculosis. METHODS The validity of...

A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient

Background and Purpose: Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis an...

Evaluation of COBAS TaqMan real time PCR assay for the diagnosis of Mycobacterium tuberculosis.

a real time pcr assay on blood for diagnosis of invasive candidiasis in immunocompromised patient

background and purpose: invasive candidiasis (ic) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent ic progress is necessary. we established a real-time pcr assay on blood for the diagnosis an...

A Real-Time RT-PCR Assay for Genotyping of Rotavirus Strains

Background: Human rotavirus (HRV) is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on rever...